Hb%

Synonyms

   Haemoglobin percent , Hgb%

Clinical Significance

   The hemoglobin (Hgb) test measures how much hemoglobin in red blood cells. Hb is the iron-containing oxygen-transport metalloprotein in red blood cells (erythrocytes). Hemoglobin in blood carries oxygen from the respiratory organs to the rest of the body tissues. Haemoglobin bound to oxygen give RBCs their chrom color, therefore variation in haemoglobin are referred to as chromic changes.

Specimen

   Whole blood 1ml or 3ml

Stability

   24 hours at 20 - 25 ˚C

   72 hours at 2 to 8 ˚C

   7 days at -20 ˚C

Reference Range

   Male : 12.5 – 16.0 g/dL

   Female : 11.5 – 15.0 g/dL

Interpretation

   Haemoglobin is decreased in:

       Blood loss

       Protein malnutrition

       Iron deficiency

       Copper deficiency

       Vitamin C deficiency

       Vitamin B1 deficiency

       Vitamin B12 deficiency

       Folic Acid deficiency

       Chronic liver disease

       Chronic kidney disease

       Bone marrow insufficiency

       Worm infestation

       Haemodilution in pregnancy

       Herediatary anemia

   Haemoglobin is increased in:

       Polycythemia vera

       High altitude adaptation

       Pulmonary pathology

       Spenic hypofunction

       Testosterone supplementation

       Dehydration

       Emphysema

       Severe asthma

       Long standing respiratory distress

       Macrocytosis

       Adrenal cortex overactivity

Limitation

   There are a number of adverse effects associated with hemoglobin. This happens either when the hemoglobin levels are high because there are too many red blood cells in the bloodstream, or when larger amounts of free hemoglobin are released into the blood flow because of increased red blood cell destruction. Treatment with antibiotics, anti-inflammatory and other drugs, such as cotrimoxazole, ciprofloxacin, lorazepam, and diclofenac can also cause releasing a massive amount of free hemoglobin into the bloodstream. If hemoglobin level is below or above normal, it needs to evaluate the hemoglobin test results along with those of other tests, or additional tests may be necessary, to determine the real treatment.